quantitation相关论文
The present study was designed to develop a sensitive and selective specific high performance liquid chromatography (HPL......
LC-MS/MS is a powerful analytical tool for the analysis of polar,semi-volatile,and thermally labile compounds of a wide ......
艾塞那肽是首个被开发的用于治疗2型糖尿病的胰岛糖素样肽1 (GLP-1)受体的激动剂.现今艾塞那肽的体内定量分析多采用酶联免疫法,但......
Targeted LC-MS based assays are increasingly applied in the post-discovery omics area with emphasis on validation,the fi......
It is well established that the C.elegans insulin/IGF-1 receptor tyrosine kinase DAF-2 acts though a highly conserved ki......
Quantitative Analyses of Chiral Isomers (Eipgoitrin/Goitrin) from Isatis Indigotica Fort Root Extrac
Traditional Chinese Medicines (TCMs) have played an important role in Asia as major clinical therapies for thousands of ......
Qualitative and Quantitative Characterization of a NovelDIA Method for Omics Analysis and its Applic
Quadrupole time of flight(Q-ToF)MS is a well-established tool for both discovery and quantitative applications.Here we d......
Rapid Determination of Compositions of Blended Oils by Matrix-assisted Laser Desorption/Ionization M
Different edible oil species have different triacylglycerols(TAGs)patterns which can be used as fingerprints for authent......
How to use open-pFind in deep proteomics data analysis?-A protocol for rigorous identification and q
High-throughput proteomics based on mass spectrometry(MS)analysis has permeated biomedical science and propelled numerou......
With the enhancement of environmental awareness,VOC-free coalescent agents attract more attentions.However,some conc......
Mitochondria are the important organelles that affect tumor initiation,growth,survival,and metastasis.The mitochondr......
A standardized method to create peripheral nerve injury in dogs using an automatic non-serrated forc
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Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS
The roots of O. fragrans are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/......
A 3-D morphological approach on spatial form and cultural identity of ethnic mountain settlements:Ca
Ethnic mountain settlements are living heritage of varied vernacular cultures.The preservation of both the built form an......
Preparation and Application of Silica-based Perfusion Packing of Size Exclusion Chromatography for Q
The synthesis and characterization of a novel macroporous silica derived size exclusion chromatography (SEC) packing for......
Synthesis of Well-defined Poly(tetrahydrofuran)-b-Poly(α-amino acid)s via Cationic Ring-opening Poly
The synthesis of block copolymers of poly(tetrahydrofuran)-b-poly(a-amino acid) (PTHF-b-PAA) is challenging since it is ......
Ultra?High?Performance Liquid Chromatograph with Triple?Quadrupole Mass Spectrometer Quantitation of
Objective: The study objective was to determine phenolic components for the quality control (QC) of cultivated Sarcandra......
通过液相色谱法、核磁共振法、红外光谱法、质谱法对DCJTB产品进行定性检测,结果表明目标产物是trans-DCJTB,关键杂质是cis-。HPLC......
Preparation and Application of Silica-based Perfusion Packing of Size Exclusion Chromatography for Q
The synthesis and characterization of a novel macroporous silica derived size exclusion chromatography (SEC) packing for......
目的 应用AutoCAD计算机辅助设计软件,建立定量分析牙槽骨水平的新方法并对其进行评价.方法 用AutoCAD软件在数字曲面体层片上分别......
以已知浓度的柠檬酸作为定量外标,琥珀酸和烟酰胺分别作为定量内标,建立了食品中水苏糖的核磁共振定量分析检测方法。该方法在0.02......
乙型肝炎病毒表面抗原(HBsAg)定量检测是临床治疗乙型肝炎的一个非常重要的指标,它不仅能区分乙型肝炎病程的各种分期,而且对药物治......
Objective The aim of the study was to investigate the expression of proteins in colonic tissues of mice with ulcerative ......
从储层岩性、成岩作用、隔夹层研究等方面入手,利用渗透率变异系数、突进系数、级差、隔夹层参数以及孔隙度、渗透率的变化对桩74块......
用PCR技术从一临床病猪组织中扩增出猪圆环病毒2型(PCV2)417 bp片段,并克隆到pGEM-T Easy载体中,构建成含有PCV2基因片段的重组质粒......
前人对地层结垢的研究大多属于定性分析,很少有定量研究。文中以绥中36-1油田某区块为研究对象,在详细分析地层水扣注入水离子质量浓......
本文在Fu等(1996)和Bean(1998)蛋白提取方法的基础上,对面粉细度、提取温度、蛋白酶抑制剂以及正丙醇浓度等方面进行了研究,建立了一......
目的测定首批对照品头孢孟多酯钠含量和头孢孟多校正因子.方法采用LC/MSNMR的方法鉴定了样品中所含的主要降解产物为头孢孟多;选择......
目的建立抗戊型肝炎病毒(Anti-HEV)IgG抗体的定量线性标准品,并进行初步应用。方法利用抗-HEV IgG和抗-HEV IgM ELISA检测试剂筛选出......
目的对检测轮状病毒滴度的荧光灶试验(FFA)方法进行改进。方法以FFA法检测轮状病毒滴度,并观察残留血清、病毒吸附量、甲基纤维素、离......
用FⅧ相关抗原免疫组化染色定量观察48例乳腺浸润癌。结果表明:有腋窝淋巴结转移组血管密度为每mm^2135、4±47.8个,无腋窝淋巴结转移组血管密度......
为探讨巴马香猪CYP3A29 mRNA的组织分布特征,全面比较其与人CYP3A4 mRNA组织分布规律,利用TaqMan RT—PCR技术对巴马香猪主要组织CYP......
结合外标法或同位素稀释质谱法,建立UPLC-Q-TOF快速定量氨基酸分析方法。在无离子对试剂及衍生化情况下,实现对样品中氨基酸的快速......
[目的]探讨乙型肝炎患者血清中HBV DNA含量与HBV血清学标志的关系。[方法]测定不同乙肝血清学标志模式和247份标志物全阴性的临床......
简要介绍了食用植物油中氮气含量定量检测装置及方法。通过该检测装置可测定出不同种类食用植物油在8~35℃的饱和氮气含量,以饱和......
建立了高效液相色谱-紫外法测定苦楝皮中成分阿魏酸含量的方法。采用MicrosorbC18色谱柱(4.6mm×250mm,5μm),以甲醇-水-磷酸(24:76:0.1......
地物光谱仪定标对成像光谱遥感定量化研究是很必要的,通过SE590地物光谱仪实验室光谱定标和辐射定标发现其波长偏移平均达8nm,其线性响应对接......
采用近红外漫反射光谱法和偏最小二乘回归算法建立了2种测样方式对不同厂家注射用头孢哌酮钠中头孢哌酮的含量进行快速测定的定量......
本文报道采用非同位素Digoxigenin标记探针定量检测血清HBV DNA。显色膜经空气干燥,液体石蜡透明处理后,即可置酶联免疫检测仪上测定各斑点的光密度值......
利用纯化的Rubisco活化酶,制备了该酶兔抗体,并研究了该酶免疫单扩散定量技术。结果表明,在Rubisco活化酶0~200 μg/mL的浓度下,酶量与......
目的评价氪激光诱导下大鼠脉络膜新生血管(choroidal neovascularization,CNV)面积计算的可行性。方法采用氪激光击破大鼠右眼Bruch......
通过对定性、定量以及定性方法、定量方法等概念内涵的阐述,结合数学的本质特征,试图说明数学的定量和定性特别是定性特征,并对其在管......
UHPLC-ESI-HRMS Quantitation of Metabolites without Using Reference Standards: Impact of LC Flow Rate
During LC-MS quantitation of drugs for pharmacokinetic assessment, usually metabolites are not quantified due to the una......
Simultaneous Determination of Catechins and Caffeine in Green Tea-Based Beverages and Foods for Spec
Catechins in green tea have various useful features including antioxidant activity and preventive effects on metabolic s......
本文介绍了以亲和层析纯化的免抗鼠IgG类抗体及其酶标物作为捕获及检测抗体进行鼠杂交瘤培养上清及腹水中IgG类单克隆抗体的ELISA......
鞘氨醇-1-磷酸(SPP)是重要的细胞第二信使,影响细胞的生长和死亡。通过培养和收集转染SPP受体-EDG-1的HEK293细胞,与标记及非标记SPP共孵育,利用它们与HEK293细胞的竞争......
The advantages of combining qualitative and quantitative analysis on a single analytical technique have further extended......
The advantages of combining qualitative and quantitative analysis on a single analytical technique have further extended......
Rapid Quantitative Determination of Isoprene Monomer in Living Taraxacum kok-saghyz by Ultra-High Pe
Taraxacum kok-saghyz(TKS)is rich in natural rubber(NR),a natural organic macromolecular compound composed of cis-1,4-pol......
Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Re
The textile industrial chain all over the world is facing a challenge of differentiating cashmere fiber from mixture of ......